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human umbilical cord vascular endothelium cells  (ATCC)


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    ATCC human umbilical cord vascular endothelium cells
    Human Umbilical Cord Vascular Endothelium Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1614 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+umbilical+cord+vascular+endothelium+cells/HUV-EC-C/10__1002_slash_adfm__202206620-317-0-8
    Average 96 stars, based on 1614 article reviews
    human umbilical cord vascular endothelium cells - by Bioz Stars, 2026-10
    96/100 stars

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    human umbilical cord vascular endothelium cells  (ATCC)
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    A - Representative images of <t>HUVEC</t> (nuclei in cyan, F-actin in magenta) treated with PBS or EVh on a gelatin coating (gray), with degradation spots in black (scale bar: 50 µm). B – Quantification of degraded gelatin area (µm 2 ) in individual HUVEC cultures. C – Cell circularity index for quantification of HUVEC morphology in individual culture. D - Tracking of HUVEC trajectory over 24h. E – Average track speed of cells in 24 h (µm/s). F - Total distance traveled by cells in 24 h (µm). G – Experimental design of the indirect co-culture assay, created in BioRender.com. H – Representative images of HUVEC (nuclei in cyan, F-actin in yellow; scale bar: 50 µm) in indirect co-culture with MDA-MB-231 (nuclei in cyan, F-actin in magenta; scale bar: 100 µm). I – Cell circularity index for quantification of HUVEC and MDA-MB-231 morphology in direct co-culture. J – Representative images of gelatin matrix (gray) with degradation spots identified by white arrows. K - MMP-2 detected by gelatin zymography and densitometry analysis to identify gelatinase activity in indirect co-culture. L – Quantification of degraded gelatin area (µm 2 ) in indirect co-culture. M - Experimental design of the direct co-culture assay, created in BioRender.com. N – Representative images of HUVEC (cytoplasm in yellow) in direct co-culture with MDA-MB-231 (nuclei in cyan, F-actin in magenta) seeded atop a gelatin coating (gray) (scale bar: 100 µm). O - Cell circularity index for quantification of combined cell morphology in direct co-culture. P - Quantification of degraded gelatin area (µm 2 ) in direct co-culture. Q – Cell viability of HUVEC and MDA-MB-231 direct co-culture. Assays were repeated on three different occasions with technical triplicates. p values indicated above comparative bars with statistical significance.
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    umbilical cord endothelial cells  (ATCC)
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    A - Representative images of <t>HUVEC</t> (nuclei in cyan, F-actin in magenta) treated with PBS or EVh on a gelatin coating (gray), with degradation spots in black (scale bar: 50 µm). B – Quantification of degraded gelatin area (µm 2 ) in individual HUVEC cultures. C – Cell circularity index for quantification of HUVEC morphology in individual culture. D - Tracking of HUVEC trajectory over 24h. E – Average track speed of cells in 24 h (µm/s). F - Total distance traveled by cells in 24 h (µm). G – Experimental design of the indirect co-culture assay, created in BioRender.com. H – Representative images of HUVEC (nuclei in cyan, F-actin in yellow; scale bar: 50 µm) in indirect co-culture with MDA-MB-231 (nuclei in cyan, F-actin in magenta; scale bar: 100 µm). I – Cell circularity index for quantification of HUVEC and MDA-MB-231 morphology in direct co-culture. J – Representative images of gelatin matrix (gray) with degradation spots identified by white arrows. K - MMP-2 detected by gelatin zymography and densitometry analysis to identify gelatinase activity in indirect co-culture. L – Quantification of degraded gelatin area (µm 2 ) in indirect co-culture. M - Experimental design of the direct co-culture assay, created in BioRender.com. N – Representative images of HUVEC (cytoplasm in yellow) in direct co-culture with MDA-MB-231 (nuclei in cyan, F-actin in magenta) seeded atop a gelatin coating (gray) (scale bar: 100 µm). O - Cell circularity index for quantification of combined cell morphology in direct co-culture. P - Quantification of degraded gelatin area (µm 2 ) in direct co-culture. Q – Cell viability of HUVEC and MDA-MB-231 direct co-culture. Assays were repeated on three different occasions with technical triplicates. p values indicated above comparative bars with statistical significance.
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    Image Search Results


    A - Representative images of HUVEC (nuclei in cyan, F-actin in magenta) treated with PBS or EVh on a gelatin coating (gray), with degradation spots in black (scale bar: 50 µm). B – Quantification of degraded gelatin area (µm 2 ) in individual HUVEC cultures. C – Cell circularity index for quantification of HUVEC morphology in individual culture. D - Tracking of HUVEC trajectory over 24h. E – Average track speed of cells in 24 h (µm/s). F - Total distance traveled by cells in 24 h (µm). G – Experimental design of the indirect co-culture assay, created in BioRender.com. H – Representative images of HUVEC (nuclei in cyan, F-actin in yellow; scale bar: 50 µm) in indirect co-culture with MDA-MB-231 (nuclei in cyan, F-actin in magenta; scale bar: 100 µm). I – Cell circularity index for quantification of HUVEC and MDA-MB-231 morphology in direct co-culture. J – Representative images of gelatin matrix (gray) with degradation spots identified by white arrows. K - MMP-2 detected by gelatin zymography and densitometry analysis to identify gelatinase activity in indirect co-culture. L – Quantification of degraded gelatin area (µm 2 ) in indirect co-culture. M - Experimental design of the direct co-culture assay, created in BioRender.com. N – Representative images of HUVEC (cytoplasm in yellow) in direct co-culture with MDA-MB-231 (nuclei in cyan, F-actin in magenta) seeded atop a gelatin coating (gray) (scale bar: 100 µm). O - Cell circularity index for quantification of combined cell morphology in direct co-culture. P - Quantification of degraded gelatin area (µm 2 ) in direct co-culture. Q – Cell viability of HUVEC and MDA-MB-231 direct co-culture. Assays were repeated on three different occasions with technical triplicates. p values indicated above comparative bars with statistical significance.

    Journal: bioRxiv

    Article Title: Tumoral hypoxic extracellular vesicles create a protective microenvironment for triple-negative breast cancer

    doi: 10.1101/2024.11.01.621519

    Figure Lengend Snippet: A - Representative images of HUVEC (nuclei in cyan, F-actin in magenta) treated with PBS or EVh on a gelatin coating (gray), with degradation spots in black (scale bar: 50 µm). B – Quantification of degraded gelatin area (µm 2 ) in individual HUVEC cultures. C – Cell circularity index for quantification of HUVEC morphology in individual culture. D - Tracking of HUVEC trajectory over 24h. E – Average track speed of cells in 24 h (µm/s). F - Total distance traveled by cells in 24 h (µm). G – Experimental design of the indirect co-culture assay, created in BioRender.com. H – Representative images of HUVEC (nuclei in cyan, F-actin in yellow; scale bar: 50 µm) in indirect co-culture with MDA-MB-231 (nuclei in cyan, F-actin in magenta; scale bar: 100 µm). I – Cell circularity index for quantification of HUVEC and MDA-MB-231 morphology in direct co-culture. J – Representative images of gelatin matrix (gray) with degradation spots identified by white arrows. K - MMP-2 detected by gelatin zymography and densitometry analysis to identify gelatinase activity in indirect co-culture. L – Quantification of degraded gelatin area (µm 2 ) in indirect co-culture. M - Experimental design of the direct co-culture assay, created in BioRender.com. N – Representative images of HUVEC (cytoplasm in yellow) in direct co-culture with MDA-MB-231 (nuclei in cyan, F-actin in magenta) seeded atop a gelatin coating (gray) (scale bar: 100 µm). O - Cell circularity index for quantification of combined cell morphology in direct co-culture. P - Quantification of degraded gelatin area (µm 2 ) in direct co-culture. Q – Cell viability of HUVEC and MDA-MB-231 direct co-culture. Assays were repeated on three different occasions with technical triplicates. p values indicated above comparative bars with statistical significance.

    Article Snippet: Dermal fibroblasts HDFa (ATCC ® PCS-201-012 TM ) and umbilical cord endothelial cells HUVEC (ATCC ® CRL-1730 TM ) were maintained between passages 6 and 15 in DMEM 10% FBS 1% pen/strep at 37 °C, 5% CO 2 .

    Techniques: Co-culture Assay, Co-Culture Assay, Zymography, Activity Assay

    A - Representative images of HDFa (nuclei in cyan, F-actin in magenta) treated with PBS or EVh on a gelatin coating (gray), with degradation spots identified by white arrows (scale bar: 100 µm). B – Cell circularity index for quantification of HDFa morphology in individual culture. C - Quantification of degraded gelatin area (µm 2 ) in individual HUVEC cultures. D – HDFa cell viability in individual culture. E - Tracking of HDFa trajectory over 24h. F – Average track speed of cells in 24 h (µm/s). G - Total distance traveled by cells in 24 h (µm). H – Representative images of HDFa (nuclei in cyan, F-actin in yellow; scale bar: 100 µm) in indirect co-culture with MDA-MB-231 (nuclei in cyan, F-actin in magenta; scale bar: 30 µm). I – Cell circularity index for quantification of HDFa and MDA-MB-231 morphology in indirect co-culture. J - Representative images of gelatin matrix (gray) with degradation spots identified by white arrows. K - Quantification of degraded gelatin area (µm 2 ) in indirect co-culture. L – MMP-2 detected by gelatin zymography and densitometry analysis to identify gelatinase activity in indirect co-culture. M – Representative images of MDA-MB-231 (cytoplasm in yellow) in direct co-culture with HDFa (nuclei in cyan, F-actin in magenta) seeded atop a gelatin coating (gray) (scale bar: 100 µm). N - Cell circularity index for quantification of combined cell morphology in direct co-culture. O – Quantification of degraded gelatin area (µm 2 ) in direct co-culture. Assays were repeated on three different occasions with technical triplicates. p values indicated above comparative bars with statistical significance.

    Journal: bioRxiv

    Article Title: Tumoral hypoxic extracellular vesicles create a protective microenvironment for triple-negative breast cancer

    doi: 10.1101/2024.11.01.621519

    Figure Lengend Snippet: A - Representative images of HDFa (nuclei in cyan, F-actin in magenta) treated with PBS or EVh on a gelatin coating (gray), with degradation spots identified by white arrows (scale bar: 100 µm). B – Cell circularity index for quantification of HDFa morphology in individual culture. C - Quantification of degraded gelatin area (µm 2 ) in individual HUVEC cultures. D – HDFa cell viability in individual culture. E - Tracking of HDFa trajectory over 24h. F – Average track speed of cells in 24 h (µm/s). G - Total distance traveled by cells in 24 h (µm). H – Representative images of HDFa (nuclei in cyan, F-actin in yellow; scale bar: 100 µm) in indirect co-culture with MDA-MB-231 (nuclei in cyan, F-actin in magenta; scale bar: 30 µm). I – Cell circularity index for quantification of HDFa and MDA-MB-231 morphology in indirect co-culture. J - Representative images of gelatin matrix (gray) with degradation spots identified by white arrows. K - Quantification of degraded gelatin area (µm 2 ) in indirect co-culture. L – MMP-2 detected by gelatin zymography and densitometry analysis to identify gelatinase activity in indirect co-culture. M – Representative images of MDA-MB-231 (cytoplasm in yellow) in direct co-culture with HDFa (nuclei in cyan, F-actin in magenta) seeded atop a gelatin coating (gray) (scale bar: 100 µm). N - Cell circularity index for quantification of combined cell morphology in direct co-culture. O – Quantification of degraded gelatin area (µm 2 ) in direct co-culture. Assays were repeated on three different occasions with technical triplicates. p values indicated above comparative bars with statistical significance.

    Article Snippet: Dermal fibroblasts HDFa (ATCC ® PCS-201-012 TM ) and umbilical cord endothelial cells HUVEC (ATCC ® CRL-1730 TM ) were maintained between passages 6 and 15 in DMEM 10% FBS 1% pen/strep at 37 °C, 5% CO 2 .

    Techniques: Co-Culture Assay, Zymography, Activity Assay

    A – Experimental design of the multicellular circulating co-culture assay (MC-CC) using the QV500 system (Kirstall). B - Representative images of MC-CC chambers containing MDA-MB-231, HUVEC or HDFa treated with PBS or EVh. (scale bars: 30 µm and 50 µm). C - Quantification of degraded gelatin area (µm 2 ) in the MC-CC MDA-MB-231 chamber. D – Band densitometry of MMP-2 detected by gelatin zymography from the MC-CC conditioned media. E,F – Cytokines IL-1β and IL-6 probed from the MC-CC conditioned media by ELISA. G,H – Relative fluorescence intensity of β-catenin and VEGFR2 in HUVEC, respectively. I,J – Relative fluorescence intensity of collagen II and III in HDFa, respectively. Assays were repeated on four different occasions with a single replicate. p values indicated above comparative bars with statistical significance.

    Journal: bioRxiv

    Article Title: Tumoral hypoxic extracellular vesicles create a protective microenvironment for triple-negative breast cancer

    doi: 10.1101/2024.11.01.621519

    Figure Lengend Snippet: A – Experimental design of the multicellular circulating co-culture assay (MC-CC) using the QV500 system (Kirstall). B - Representative images of MC-CC chambers containing MDA-MB-231, HUVEC or HDFa treated with PBS or EVh. (scale bars: 30 µm and 50 µm). C - Quantification of degraded gelatin area (µm 2 ) in the MC-CC MDA-MB-231 chamber. D – Band densitometry of MMP-2 detected by gelatin zymography from the MC-CC conditioned media. E,F – Cytokines IL-1β and IL-6 probed from the MC-CC conditioned media by ELISA. G,H – Relative fluorescence intensity of β-catenin and VEGFR2 in HUVEC, respectively. I,J – Relative fluorescence intensity of collagen II and III in HDFa, respectively. Assays were repeated on four different occasions with a single replicate. p values indicated above comparative bars with statistical significance.

    Article Snippet: Dermal fibroblasts HDFa (ATCC ® PCS-201-012 TM ) and umbilical cord endothelial cells HUVEC (ATCC ® CRL-1730 TM ) were maintained between passages 6 and 15 in DMEM 10% FBS 1% pen/strep at 37 °C, 5% CO 2 .

    Techniques: Co-culture Assay, Zymography, Enzyme-linked Immunosorbent Assay, Fluorescence

    A – Representative images of THP-1 (magenta, stained with CellTracker CMPTX) attachment to MDA-MB-231 MC-CC chambers (nuclei in cyan, F-actin in gray; scale bar: 30 µm). B - Representative images of THP-1 cells (magenta, stained with CellTrace CSFE) in direct co-culture with MDA-MB-231, HUVEC or HDFa (nuclei in cyan, F-actin in gray; scale bars: 50 µm). C - Cell circularity index for quantification of combined cell morphology in THP-1 direct co-culture with MDA-MB-231, HUVEC and HDFa. D – Quantification of degraded gelatin area (µm 2 ) in THP-1 direct co-culture with MDA-MB-231, HUVEC and HDFa. Assays were repeated on three different occasions with technical triplicates. p values indicated above comparative bars with statistical significance.

    Journal: bioRxiv

    Article Title: Tumoral hypoxic extracellular vesicles create a protective microenvironment for triple-negative breast cancer

    doi: 10.1101/2024.11.01.621519

    Figure Lengend Snippet: A – Representative images of THP-1 (magenta, stained with CellTracker CMPTX) attachment to MDA-MB-231 MC-CC chambers (nuclei in cyan, F-actin in gray; scale bar: 30 µm). B - Representative images of THP-1 cells (magenta, stained with CellTrace CSFE) in direct co-culture with MDA-MB-231, HUVEC or HDFa (nuclei in cyan, F-actin in gray; scale bars: 50 µm). C - Cell circularity index for quantification of combined cell morphology in THP-1 direct co-culture with MDA-MB-231, HUVEC and HDFa. D – Quantification of degraded gelatin area (µm 2 ) in THP-1 direct co-culture with MDA-MB-231, HUVEC and HDFa. Assays were repeated on three different occasions with technical triplicates. p values indicated above comparative bars with statistical significance.

    Article Snippet: Dermal fibroblasts HDFa (ATCC ® PCS-201-012 TM ) and umbilical cord endothelial cells HUVEC (ATCC ® CRL-1730 TM ) were maintained between passages 6 and 15 in DMEM 10% FBS 1% pen/strep at 37 °C, 5% CO 2 .

    Techniques: Staining, Co-Culture Assay